Title

Measuring LRRK2 Levels in The Ventral Midbrain in a G2019S mutant LRRK2-associated Parkinson’s Disease Mouse Model

Creator

Samlian, Miranna

Abstract

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of motor control and dopaminergic neurons in the substantia nigra pars compacta region in the ventral midbrain. Only 5-10% of PD cases are familial, with the most common dominantly-inherited gene being LRRK2. LRRK2 holds a high potential as a PD biomarker due to its hyperactive kinase function associated with idiopathic PD and mutant LRRK2-induced PD, where G2019S is the most common pathogenic mutation.

Methodology: In-house Sandwich ELISA was used to measure total LRRK2, flag-tagged LRRK2, and
phosphorylated LRRK2 levels in the ventral midbrains of mice 3-weeks post-injection with helpful adenovirus vectors to induce LRRK2 expression.

Results: Differences were observed in LRRK2 levels between treatment groups, however they were found to be insignificant 3-weeks post-injection. For future research, larger cohorts and measuring LRRK2 levels at later time-points should be considered.

Keywords

parkinson's disease

elisa

lab-work

Subject

biomedical sciences

Date Available

June 10, 2024

Type

Poster

Course

BMS 4650 Capstone in Biomedical Sciences

Instructor

Polissidis, Alexia

Session

Poster Display

Location

Student Lounge

Event

Year 2024 | 8th Annual Student Research and Creative Arts Symposium