Comparative Structural Analysis of Sodium Channels Using X-ray Crystallography, AlphaFold 3, Cryo-EM, and Ligand Interactions: An Educational Perspective

Title
Comparative Structural Analysis of Sodium Channels Using X-ray Crystallography, AlphaFold 3, Cryo-EM, and Ligand Interactions: An Educational Perspective
Creator
Koranis, Athanasios
Abstract
Voltage-gated sodium channels (Nav) are crucial for neuronal excitability and action potential propagation. This study integrates X-ray crystallography, AlphaFold 3 predictions,
cryo-EM, and ligand interaction studies to analyze the generic Nav structure and function. AlphaFold 3 predicts key regions,
cryo-EM captures dynamic states, and X-ray crystallography provides atomic-level resolution. Ligand studies reveal modulation by toxins and drugs. Comparing these methods highlights their strengths in resolving structural dynamics. Integrating computational modeling, experimental validation,
and ligand studies enhances neurobiology education by bridging theoretical concepts with practical applications in protein structure-function analysis.

Authors: Athanasios Koranis, Eirini Tzermpou, Sofia Orfanou, Paraskevi
Papadopoulou
Subject
Biomedical Sciences
Date Available
May 22, 2025
Type
Group Research Project
Course
BI 3232 Cellular and Molecular Neurobiology
Instructor
Papadopoulou, Paraskevi
Session
Poster
Location
Deree Corridor
Event
Year 2025 | 9th Annual Student Research and Creative Arts Symposium

Tags

Biomedical Sciences

Tags

Biomedical Sciences