Title

Automated Image Analysis of Neuroinflammation: staging microglia activation in
the brain

Creator

Tzermpou, Eirini Maria

Abstract

This work, produced within the framework of the Biomedical Sciences Capstone course, presents a novel way to cluster, classify and detect microglia activation in mice brain slices with the use of confocal and fluorescence lifetime imaging
microscopy (FLIM). By collecting multi-modal data from the acute murine viral model of G2019S and WT LRRK2, we proposed a continuous microglia activation scale additional to the already existing multiclass microglia activation grouping,
with the use of lifetime data and 3D cell images.

Keywords

neuroinflammation

machine learning

fluorescence lifetime imaging microscopy

Subject

Biomedical Sciences

Date Available

May 22, 2025

Type

Poster

Course

BMS 4650 Capstone in Biomedical Science

Instructor

Polissidis, Alexia Victoria

Session

Poster Session

Location

Deree Corridor

Event

Year 2025 | 9th Annual Student Research and Creative Arts Symposium