Title

Antioxidant Evaluation of Hydroxylated Benzimidazole and Curcumin Analogues Using DPPH, ABTS, and FRAP Assays

Creator

Naksi, Eleni

Abstract

"Oxidative stress significantly contributes to the development of multiple diseases, which emphasizes the importance of identifying effective antioxidant agents. In this study, antioxidant activity of eight structurally related compounds was assessed using three alternative in vitro assays, including DPPH radical scavenging, ABTS radical cation decolorization, and ferric reducing antioxidant power (FRAP). These compounds were natural curcumin, the analogues of curcuminoids, and benzimidazole compounds. The experiments were carried out in 96-well plates which contained negative and positive controls. Serial dilutions were done to assess responses that were concentration dependent. Compounds with free phenolic hydroxyl groups by hydrogen atom transfer (HAT) mechanisms displayed strong radical-scavenging activity in DPPH and ABTS assays and the findings showed strong structure activity relationships. Electron-donating functional groups, like dimethylamino groups, in compounds containing neither phenolic groups, led to higher reducing power in the FRAP assay, indicating that one-electron transfer (SET) mechanism was the dominating one. Benzimidazole derivatives were found to be mostly dependent on the number and location of phenolic functionalities as opposed to the heterocyclic core itself. In general, the findings indicate that even a slight structural modification can significantly impact the antioxidant behaviour and the paper has highlighted the importance of applying a diversity of methods to determine antioxidant capacities."

Keywords

antioxidant

benzimidazole

free radicals

Subject

Biomedical Science

Date Available

May 27, 2026

Type

Poster

Course

BMS 4750 Capstone in Biomedical Sciences

Instructor

Kythreoti, Georgia

Session

Poster

Location

Student Lounge

Event

Year 2026 | 10th Annual Student Research and Creative Arts Symposium