Title

Study of the Genotoxicity Potential of Creatine Monohydrate Using Human Osteosarcoma Cells

Creator

Schmidt, Anna Nikolett

Abstract

"Creatine monohydrate is a widely used supplement for enhancing athletic performance. While generally considered safe, concerns have been raised about its potential to promote carcinogenic compounds. This study investigated its cyto- and genotoxic effects on human osteosarcoma (U2OS) cells.
Cytotoxicity, assessed using the Trypan Blue assay, showed >98% cell viability, indicating no toxic effects. Genotoxicity was evaluated using γH2AX immunofluorescence to detect DNA double-strand breaks. No DNA damage was observed at concentrations of 1–10 mM.
Interestingly, γH2AX staining decreased with increasing creatine concentrations, suggesting a potential genoprotective effect. At 7 mM, cells showed particularly low levels of DNA damage. Further research is needed to confirm these findings and identify optimal protective concentrations."

Keywords

immunofluorescent

creatine

genotoxicity

Subject

Biomedical Science

Date Available

May 27, 2026

Type

Poster

Course

BMS4750 Capstone in Biomedical Sciences

Instructor

Fragkos, Michalis

Session

Poster

Location

Student Lounge

Event

Year 2026 | 10th Annual Student Research and Creative Arts Symposium